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Background Balanced Salt Solution Physiological Solution have the properties of buffer solution regulate pH , normal saline maintain osmotic pressure and culture medium provide nutrition. Variable F corresponds to the flow rate of each experimental group; variable A is the dentine area exposed to the fluid; variable P is the intrapulpal pressure, which value corresponds to the height of the column with distilled water mm , and variable t is time in minutes.
Once the dentin disk image is obtained with Image J, the required area is selected and the requested values obtained. The groups were:. Table 1 shows the means and their respective standard deviations of the different permeability rates obtained in various storage media.
Figure 4 shows the comparison of daily averages of the 3 study groups. In order to determine which groups present significant difference, the post-hoc test is conducted figures 5 , 6 y 7. The results show significant difference p Based on the results, it is possible to observe significant differences p This study arises from the need for materials to develop short- and long-term studies on dentin permeability without altering the agents by reactions among dental tissues, materials used, and storage solution.
The alternatives currently tested have not proven to provide all of these conditions, since some agents such as saline solutions and other similar solutions have failed to demonstrate their usefulness as storage agents in the mid-term and others, such as formalin, have shown better results but they alter tissue characteristics that may eventually affect the results.
The model used for this study is based on that used by the research group led by Pashley 31 and other researchers afterwards. This model offers a simple, reliable tool: the diffusion chamber that allows to visually measure dentin disks' hydraulic conductance and to determine dentin permeability. Based on the technique used, we may establish a comparison with Sekimoto, who used the same model but with a pressure of 15 psi of N2 on a 0.
In this case, and because of possible future uses of this study, we decided to use a pressure close to physiological pulpal pressure which according to several authors ranges between 12 and 18 cm H 2 O. The fact that dentin flow tends to move from the pulp towards the tooth's external portion is due to the difference in pressure between the environment and the pulp; since the latter is higher, it generates an expulsive flow of fluid from the pulp.
Nevertheless, the variation found by this author between both flow directions was not statistically significant. Similarly, permeability rate was measured by means of distilled water instead of serum in order to avoid precipitation of salts, which may obliterate dentinal tubules. Hank's solution was used in this study with the intention of providing dentin permeability studies with a solution that has shown to maintain dentinal tissue over time, as it retains much of its mechanical, chemical, and even physiological properties.
All this is due in large part to its similarities with tooth mineral phases, both in pH and osmolarity, besides sharing similar ions with them. The results of this study allow us to infer some facts. Similarly, and considering figure 4 , the group that showed the highest increases, particularly during the first days of study, was that of the samples stored in saline solution.
For Goodis et al, 28 this is probably due to the fixative property of formalin, which retains the dentinal tubules structure, producing smaller hydraulic conductance changes. These results show significant differences p We may conclude that the agents under study present significant differences among themselves in some moments of the analysis but they cannot be comparable, at least in terms of hydraulic conductance, to the fact of storing samples in any storage solution.
Regarding Hank's solution, no significant difference was observed among the samples during the days of study, therefore proving the null hypothesis of this study.
However, we suggest performing further studies on dentin permeability and Hank's solution's behavior as a storage agent by means of projects that include even longer times than the ones evaluated in the present study. It is also recommended to check any reaction of the material against Hank's solution before analyzing any material in this solution.
There are statistically significant differences among the various types of dental sample storage for 4 of the 9 days when the samples' hydraulic conductance was studied days 2, 7, 14, and Dentin permeability: determinants of hydraulic conductance. J Dent Res ; 57 2 : Fluid and protein flux across the pulpodentine complex of the dog in vivo.
Arch Oral Biol ; 36 2 : Regional variability in the permeability of human dentine. Arch Oral Biol ; 32 7 : Dentin hydraulic conductance: changes produced by red blood cells.
J Dent Res ; 60 10 : Dentin permeability: effects of smear layer removal. J Prosthet Dent ; 46 5 : Dentin permeability: effects of temperature on hydraulic conductance. J Dent Res ; 62 9 : Microleakage measurement of selected restorative materials: a new in vitro method. J Prosthet Dent ; 56 4 : Pashley DH. Dentin permeability, dentin sensitivity, and treatment through tubule occlusion.
J Endod ; 12 10 : Effects of the smear layer, Copalite, and oxalate on microleakage. Oper Dent ; 11 3 : Dentin permeability and bond strengths after various surface treatments.
Dent Mater ; 5 6 : Mechanisms of dentin sensitivity. Dent Clin North Am ; 34 3 : Dentine permeability and its role in the pathobiology of dentine sensitivity. The Dentin Disc surface: a plausible model for dentin physiology and dentin sensitivity evaluation. Adv Dent Res ; 11 4 : In patients with negative scan for IBD, diagnosis was established by endoscopy.
In the remaining case, the false negative case, scans did not show any abnormal uptake while rectoscopy indicated the presence of CD. Absence of active disease was confirmed by endoscopy in 7 patients in one case one ischemic colitis was diagnosed. Active disease was confirmed by endoscopy in 14 cases, by contrast radiology in one case, by CT in one case of pancolitis and by surgery in 2 cases.
The remaining case, the false negative case, presented normal scintigrams while endoscopy showed the presence of ulcerative colitis UC lesions response to treatment. On the other hand, absence of active disease was confirmed by endoscopy in 10 patients 6 showed normal endoscopy, 3 cases had diverticulosis, one case ischemic colitis and one case had lymphadenitis. Table 2 shows the values of sensitivity, specificity, negative predictive value and accuracy for both tracers.
In 36 patients disease extension was evaluated at 6 h p. No differences were seen between early 30 min and late scan 2h ; the number of segments detected was 29 and 30 for LPP group and 19 and 22 for HBSS group. Nevertheless at 6 h p. It is important to remark that 4 cases in the LPP in and in 12 of the HBSS group the number of segments increased being positive the previous scans and more significant, in one case of LPP group and in 4 of the HBSS group, 30 min and 2 h scans were normal while the 6 h scan showed abnormal uptake, which means 5 false positive at this time.
Labelling efficiency is higher and diagnosis accuracy for IBD is similar. On the other hand the higher number of false positive cases found at 6 h p. Abrir menu Brasil. Brazilian Archives of Biology and Technology. Abrir menu. Cell labelling Leukocyte poor plasma group: 45 mL of venous blood collected over 6 mL acid citrate dextrose were obtained from 30 patients 19 men, 11 women.
Scintigraphic Imaging The first scan, early scan, was obtained min after 99mTc-HMPAO leukocytes reinjection while the second scan was obtained about 2 hrs post-injection of 99mTc-HMPAO leukocytes, finally a third anterior abdominal scan was obtained at 6 hours p. Biodistribution: Biodistribution was similar in both groups. Becker, W. Datz, F. AJR Am. Huub, J. Med , 28 , Med , 42 ,
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